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Hexarelin 100 mcg vs 200 mcg — Dose Comparison and Volume Table

Published 2026-08-17 · Lyophile Editorial

Reconstituting a 5 mg vial of hexarelin? The draw volume for 100 mcg or 200 mcg per injection depends entirely on how much bacteriostatic water you add. On a U-100 insulin syringe, 100 mcg requires 2 units at 1 mL reconstitution, 4 units at 2 mL, or 6 units at 3 mL — and 200 mcg is exactly double: 4, 8, or 12 units respectively. The full dose table, step-by-step math, and shots-per-vial count for both dose levels are below, so you can pick your reconstitution volume based on syringe readability rather than guesswork.

For research use only — not clinical guidance.

How much bacteriostatic water for a 5 mg hexarelin vial?

Add 1 mL, 2 mL, or 3 mL of bacteriostatic water to a 5 mg vial. All three volumes work, but the choice changes your concentration and therefore your draw volume per dose:

| Diluent volume | Concentration | |---|---| | 1 mL | 5000 mcg/mL | | 2 mL | 2500 mcg/mL | | 3 mL | 1666.7 mcg/mL |

Each concentration maps to a different number of units on a U-100 insulin syringe, where 1 unit = 0.01 mL.

The trade-off: more diluent means larger, easier-to-read draw volumes but the same total shots per vial; less diluent means smaller draws that are harder to measure precisely. For 100 mcg, 2 mL reconstitution gives a 4-unit draw — readable without a magnifier — while 1 mL gives a 2-unit draw that's easy to misread by half a unit, which is a 25 mcg error. Per USP <797> guidance on sterile compounding, bacteriostatic water (0.9% benzyl alcohol) is appropriate for multi-dose use, but the vial should be discarded 28 days after first puncture.

Hexarelin dose and volume table for a 5 mg vial

| Reconstitution volume | Concentration | 100 mcg draw (U-100 units) | 100 mcg draw (mL) | 200 mcg draw (U-100 units) | 200 mcg draw (mL) | Shots at 100 mcg | Shots at 200 mcg | |---|---|---|---|---|---|---|---| | 1 mL | 5000 mcg/mL | 2 units | 0.02 mL | 4 units | 0.04 mL | 50 | 25 | | 2 mL | 2500 mcg/mL | 4 units | 0.04 mL | 8 units | 0.08 mL | 50 | 25 | | 3 mL | 1666.7 mcg/mL | 6 units | 0.06 mL | 12 units | 0.12 mL | 50 | 25 |

Shots per vial math: 5 mg = 5000 mcg. 5000 ÷ 100 = 50 doses at 100 mcg. 5000 ÷ 200 = 25 doses at 200 mcg. Independent of reconstitution volume — the volume only changes the syringe draw, not the total peptide mass.

How to calculate hexarelin draw volume step by step

Formula: Target dose (mcg) ÷ concentration (mcg/mL) = volume (mL). Then convert to syringe units by dividing by 0.01 mL/unit.

Worked example — 200 mcg dose, 2 mL reconstitution:

For 3 mL reconstitution:

Inverse check: 12 units × 0.01 mL/unit × 1666.7 mcg/mL = 200 mcg — the math closes.

Common failure mode: Using a U-40 syringe instead of U-100. U-40 syringes are calibrated for 40 units per mL, so 8 units on a U-40 delivers 0.2 mL — five times the intended volume. Always verify your syringe barrel is marked U-100 before drawing.

Which reconstitution volume is best for hexarelin?

2 mL of bacteriostatic water is the practical sweet spot for a 5 mg vial. It yields a 4-unit draw for 100 mcg and an 8-unit draw for 200 mcg — both cleanly readable on standard insulin syringes.

Reconstitution detail: Hexarelin is a small synthetic ghrelin analog and dissolves readily in bacteriostatic water at these concentrations. Swirl gently rather than shake — vigorous agitation can denature the peptide. Let the vial sit for a few minutes after adding water to allow foam to settle before drawing. Per USP <71> sterility test guidance, the bacteriostatic water is preserved against bacterial growth, but the peptide solution is only as clean as your technique — wipe the vial stopper with an alcohol pad before each draw.

Hexarelin 100 mcg vs 200 mcg: what's the practical difference?

200 mcg doubles the peptide mass delivered per injection versus 100 mcg, which halves the number of doses per vial — 50 versus 25 — and doubles the draw volume on any syringe. At 2 mL reconstitution, that's 4 units versus 8 units on a U-100 syringe. The larger draw is easier to measure accurately, which matters more at lower concentrations where a one-unit error is a bigger percentage of the dose.

A 5 mg vial reconstituted with 2 mL gives you 2500 mcg/mL, so each unit on the syringe carries 25 mcg of hexarelin. That means:

The arithmetic scales linearly — you can interpolate any intermediate dose from the same concentration. Just remember the concentration changes if you reconstitute with a different volume, so recalculate before you draw, not after.

Hexarelin dose calculator reference

| Target dose (mcg) | 1 mL recon (units) | 2 mL recon (units) | 3 mL recon (units) | |---|---|---|---| | 50 | 1 | 2 | 3 | | 100 | 2 | 4 | 6 | | 150 | 3 | 6 | 9 | | 200 | 4 | 8 | 12 | | 250 | 5 | 10 | 15 |

Each unit on a U-100 syringe is 0.01 mL, so the volume column is just the unit count divided by 100. Per-unit peptide mass: at 1 mL reconstitution, one unit delivers 50 mcg; at 2 mL, 25 mcg; at 3 mL, approximately 16.7 mcg. If you're dosing below 100 mcg, the 2 mL or 3 mL reconstitution volumes give you more syringe resolution — a 50 mcg dose at 1 mL recon is a single unit, which is at the edge of practical accuracy for most insulin syringes.

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Frequently asked questions

How much bacteriostatic water should I add to a 5 mg hexarelin vial?

Add 1 mL, 2 mL, or 3 mL of bacteriostatic water to a 5 mg hexarelin vial. The choice changes concentration: 1 mL yields 5000 mcg/mL, 2 mL yields 2500 mcg/mL, and 3 mL yields 1666.7 mcg/mL. Per USP <797>, bacteriostatic water with 0.9% benzyl alcohol is appropriate for multi-dose use, with vial discard 28 days after first puncture.

How many units on a U-100 syringe for a 100 mcg hexarelin dose?

For a 100 mcg hexarelin dose, draw 2 units with 1 mL reconstitution, 4 units with 2 mL, or 6 units with 3 mL on a U-100 insulin syringe. Each unit equals 0.01 mL. The 2 mL option provides a 4-unit draw that is readable without a magnifier, reducing misread risk.

How many units on a U-100 syringe for a 200 mcg hexarelin dose?

For a 200 mcg hexarelin dose, draw 4 units with 1 mL reconstitution, 8 units with 2 mL, or 12 units with 3 mL on a U-100 insulin syringe. Each unit equals 0.01 mL. The 2 mL option yields an 8-unit draw, which is cleanly readable on standard insulin syringes.

How many shots are in a 5 mg hexarelin vial at 100 mcg or 200 mcg?

A 5 mg hexarelin vial contains 5000 mcg, providing 50 doses at 100 mcg and 25 doses at 200 mcg. This shot count is independent of reconstitution volume; the volume only changes the syringe draw, not the total peptide mass. Always verify your syringe is U-100 calibrated before drawing.