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Reconstitution math, dose tables, and tools for research peptides.

BPC-157 Reconstitution Calculator — 5mg and 10mg Vials

Published 2026-06-16 · Lyophile Editorial

For research use only — not clinical guidance.

Concentration Lookup Table

| Vial Size | Diluent Volume (mL) | Final Concentration (mcg/mL) | mcg per U-100 Unit | |-----------|---------------------|-----------------------------|---------------------| | 5 mg (5000 mcg) | 1 mL | 5000 mcg/mL | 50 mcg | | 5 mg (5000 mcg) | 2 mL | 2500 mcg/mL | 25 mcg | | 5 mg (5000 mcg) | 3 mL | 1667 mcg/mL | 16.7 mcg | | 10 mg (10,000 mcg) | 1 mL | 10,000 mcg/mL | 100 mcg | | 10 mg (10,000 mcg) | 2 mL | 5000 mcg/mL | 50 mcg | | 10 mg (10,000 mcg) | 3 mL | 3333 mcg/mL | 33.3 mcg |

Find your vial size on the left, pick your diluent volume. The right column shows mcg per unit mark on a U-100 syringe. Example: 5 mg vial + 2 mL diluent = 25 mcg per unit.

The Math: Step by Step

Step 1 — Vial mass: 5 mg = 5000 mcg. 10 mg = 10,000 mcg. (5 × 1000, 10 × 1000)

Step 2 — Diluent volume: Pick 1 mL, 2 mL, or 3 mL bacteriostatic water.

Step 3 — Concentration: Peptide mass ÷ diluent volume.

Step 4 — Per syringe unit: U-100 syringe = 100 units per 1 mL. Each unit = 0.01 mL. Divide concentration per mL by 100.

Watch out: A U-40 syringe has 40 units per mL — 2.5× more volume per unit mark. Calculate for U-100 but draw with U-40? You get 2.5× the intended peptide mass. Always check syringe markings.

Syringe Units for Common Research Volumes

Volume measurements only — not dosing recommendations.

| Target Dose (mcg) | 5 mg / 1 mL (50 mcg/unit) | 5 mg / 2 mL (25 mcg/unit) | 10 mg / 2 mL (50 mcg/unit) | 10 mg / 3 mL (33.3 mcg/unit) | |-------------------|---------------------------|---------------------------|---------------------------|-----------------------------| | 100 mcg | 2 units | 4 units | 2 units | 3 units | | 200 mcg | 4 units | 8 units | 4 units | 6 units | | 250 mcg | 5 units | 10 units | 5 units | 7.5 units | | 300 mcg | 6 units | 12 units | 6 units | 9 units | | 400 mcg | 8 units | 16 units | 8 units | 12 units | | 500 mcg | 10 units | 20 units | 10 units | 15 units |

Find target dose on the left, read across to your reconstitution ratio. Draw to that unit mark on a U-100 syringe. Example: 250 mcg from 5 mg / 2 mL = draw to 10 units.

Half-unit markings exist on some 0.5 mL syringes (50-unit capacity). Not all syringes have them — check before reconstituting. Finer control than a 1 mL syringe.

Reconstitution Procedure

  1. Wipe vial stopper with alcohol swab. Let dry 15 seconds — alcohol residue degrades peptides.
  2. Draw bacteriostatic water into a sterile syringe. Fresh syringe only — never reuse.
  3. Inject water slowly into BPC-157 vial. Aim needle at glass wall, not powder. Direct jet = foaming = denatured peptides.
  4. Swirl gently — do not shake. Shaking creates bubbles and shears peptide chains. Roll between palms until dissolved (30-60 seconds).
  5. Inspect solution. Clear and colorless. Cloudiness or particles = contamination — discard.
  6. Store at 2-8°C (36-46°F). Use within 28 days. Do not freeze — ice crystals damage peptide structure.

Why bacteriostatic water: Contains 0.9% benzyl alcohol — inhibits bacterial growth in multi-use vials. Sterile water without preservatives supports bacterial growth after puncture. USP <71> applies to both; only bacteriostatic water meets USP <51> preservative efficacy for multi-dose containers.

Common Pitfalls

Pitfall 1 — Over-diluting: 3 mL in a 5 mg vial = 1667 mcg/mL = 16.7 mcg per unit. A 250 mcg dose needs 15 units — large volume, uncomfortable for subcutaneous injection. Stick to 1-2 mL for 5 mg vials, 2-3 mL for 10 mg.

Pitfall 2 — Under-diluting: 0.5 mL in a 5 mg vial = 10,000 mcg/mL = 100 mcg per unit. A 100 mcg dose = 1 unit — nearly impossible to draw accurately. Human error at 1 unit can be ±50%. Use enough diluent to keep target doses at 5+ units.

Pitfall 3 — Wrong syringe: U-100 syringes = 100 units per mL. Tuberculin syringes = mL and fractions (0.1 mL, 0.2 mL). "10 units" means U-100. On a tuberculin syringe, 0.1 mL = 10 units on U-100 scale, but markings look different. Confusion between these is a leading cause of volume errors.

Pitfall 4 — Dead space: Standard insulin syringes have ~0.02-0.03 mL dead space in hub and needle. For 2 mL reconstitution: negligible (1-1.5%). For 0.5 mL reconstitution: 4-6% of drawn volume. Use low dead-space syringes for small volumes.

Bacteriostatic Water Sources

Look for "USP grade" in sterile multi-dose vials. Benzyl alcohol concentration must be 0.9% per USP <51>.

Storage: Unopened vials = 2-3 years at room temperature. Opened = use within 28 days. Discard if cloudy or particles appear. Never use past expiration — benzyl alcohol degrades, reducing antimicrobial efficacy.