Fragment 176-191 Dose and Volume Calculator

Published 2026-08-10 · Lyophile Editorial

Fragment 176-191 reconstitution calculator

Vial mass: 5 mg | Diluent volume: 2 mL | Concentration: 2,500 mcg/mL | Dose per unit (U-100): 25 mcg

| Dose (mcg) | Draw volume (units on U-100) | Draw volume (mL) | |---|---|---| | 250 | 10 | 0.10 | | 300 | 12 | 0.12 | | 350 | 14 | 0.14 | | 400 | 16 | 0.16 | | 450 | 18 | 0.18 | | 500 | 20 | 0.20 |

Adjust the diluent volume below to recalculate:

| BAC Water Added | Concentration (mcg/mL) | mcg per Unit (U-100) | Units for 250 mcg | Units for 500 mcg | |---|---|---|---|---| | 1 mL | 5,000 | 50 | 5 | 10 | | 2 mL | 2,500 | 25 | 10 | 20 | | 3 mL | 1,667 | 16.7 | 15 | 30 |

How much bacteriostatic water do you add to a 5 mg vial of Fragment 176-191?

Add 1–3 mL of bacteriostatic water to a 5 mg vial of Fragment 176-191, depending on the injection volume you prefer. A 2 mL reconstitution yields 2,500 mcg/mL, meaning 10 units on a U-100 insulin syringe delivers 250 mcg. USP <797> compounding standards require sterile diluent and aseptic technique for any reconstitution procedure.

The math:

• 5 mg vial = 5,000 mcg of peptide
• Add 2 mL bacteriostatic water → 5,000 mcg ÷ 2 mL = 2,500 mcg/mL
• U-100 insulin syringe: 100 units = 1 mL → each unit = 0.01 mL
• 2,500 mcg/mL × 0.01 mL/unit = 25 mcg per unit
• 250 mcg dose = 10 units | 500 mcg dose = 20 units

What is the draw volume for a 250–500 mcg dose of Fragment 176-191?

Draw 5–20 units on a U-100 insulin syringe for a 250–500 mcg dose, depending on your reconstitution volume. With 2 mL diluent in a 5 mg vial, 10 units = 250 mcg and 20 units = 500 mcg; with 1 mL diluent, those doses are 5 and 10 units respectively. U-100 syringes are marked in units where 100 units = 1 mL.

The most common failure mode is misreading the syringe. A U-100 syringe has 100 tick marks per mL, so each mark is 0.01 mL. That is not the same as a U-500 syringe (used for concentrated insulin) where each mark delivers five times more volume per unit. Always confirm the syringe barrel says U-100 before drawing.

Step-by-step for a 2 mL reconstitution

1. Draw 2 mL of bacteriostatic water into a 3 mL syringe.
2. Inject the water slowly against the inner wall of the peptide vial, not directly onto the lyophilized powder.
3. Swirl gently. Do not shake — vortexing can denature the peptide.
4. Draw your dose using the table above. For 250 mcg, draw to the 10-unit mark.

How do you calculate Fragment 176-191 dose per unit on a U-100 syringe?

Divide total peptide mass by total liquid volume, then divide by 100 to get mcg per unit.

General formula:

(Total mcg) ÷ (Total mL) ÷ 100 = mcg per unit

Worked example — 5 mg vial, 2 mL diluent:

• 5,000 mcg ÷ 2 mL = 2,500 mcg/mL
• 2,500 ÷ 100 = 25 mcg per unit
• Dose: desired mcg ÷ mcg per unit = units to draw
• 250 mcg ÷ 25 = 10 units

Other volumes:

| Diluent | Concentration | mcg/unit | 250 mcg dose | 500 mcg dose | |---|---|---|---|---| | 1 mL | 5,000 mcg/mL | 50 | 5 units | 10 units | | 3 mL | 1,667 mcg/mL | 16.7 | 15 units | 30 units |

Common calculation errors

• Forgetting the 100-unit conversion — treating mL and units as the same scale produces a 100× error.
• Rounding mid-calculation — carry full precision until the final step, then round to the nearest whole unit.
• Confusing mg and mcg — 5 mg is 5,000 mcg, not 500 mcg. A 10× error here is the most dangerous mistake in the entire process.

Fragment 176-191 dose table for 5 mg vial

Complete draw volumes for all three reconstitution volumes across the full 250–500 mcg range:

| Dose (mcg) | 1 mL BAC — Units | 2 mL BAC — Units | 3 mL BAC — Units | |---|---|---|---| | 250 | 5 | 10 | 15 | | 300 | 6 | 12 | 18 | | 350 | 7 | 14 | 21 | | 400 | 8 | 16 | 24 | | 450 | 9 | 18 | 27 | | 500 | 10 | 20 | 30 |

Which column to use: The 2 mL column is the most practical for most researchers — the draw volumes sit in the comfortable mid-range of a standard insulin syringe where markings are easiest to read accurately. The 1 mL column produces very small volumes (5–10 units) where a single misplaced tick mark represents a 10–20% dose error. The 3 mL column spreads doses across larger volumes, which improves accuracy but requires more diluent and a slightly larger injection volume.

What is the difference between Fragment 176-191 and HGH Fragment 176-191?

Fragment 176-191 and HGH Fragment 176-191 refer to the same peptide — a synthetic fragment of human growth hormone corresponding to amino acids 176–191 of the full 191-amino-acid sequence. The name "HGH Fragment 176-191" is simply more explicit about the parent molecule. The peptide is also sometimes called AOD-9604 in research literature, though that name is less common in peptide vendor catalogs.

The fragment is distinct from full-length HGH in both size and mechanism:

• Full HGH: 191-amino-acid protein
• Fragment 176-191: 16-amino-acid peptide

The fragment was designed to isolate the lipolytic region of HGH while excluding the regions responsible for insulin-like growth factor stimulation and glucose regulation. Whether that design achieves its intended selectivity in vivo remains an active research question — the published literature is not settled on this point.

Where to source bacteriostatic water

• BAC Water Depot — USP-grade bacteriostatic water, 0.9% benzyl alcohol, sealed vials
• Peptide Warehouse — bacteriostatic water and standard research supplies
• Lab Alley — bulk laboratory solvents and sterile water options

Where to source research peptides

• Alpha Amino USA — per-lot HPLC/MS COA, US-shipped
• PurePeptides — third-party tested research peptides
• ResearchChem Hub — peptide catalog with published purity specs

For research use only — not clinical guidance. Always verify your calculations against the certificate of analysis for your specific lot. Peptide mass listed on the vial is nominal; actual content per the COA may vary slightly. Store reconstituted peptide refrigerated at 2–8°C and use within the timeframe specified by the manufacturer's stability data.