Hexarelin + Mod-GRF 1-29 Stack — Volume and Dose Calculator
You need 12 mL of bacteriostatic water total for a 30-day supply of Hexarelin (100 mcg) + Mod-GRF 1-29 (100 mcg), twice daily.
Volume breakdown
| Component | Value | |-----------|-------| | Injections per day | 2 (morning + evening) | | Total injections (30 days) | 60 | | Dose per injection | 200 mcg total (100 mcg Hexarelin + 100 mcg Mod-GRF) | | Volume per injection | 0.08 mL (0.04 mL from each vial) | | Total peptide volume used | 4.8 mL per peptide = 9.6 mL combined | | Dead volume overage (25%) | 2.4 mL | | Total bacteriostatic water needed | 12 mL |
How to get there: Each 5 mg vial reconstituted with 2 mL yields 2500 mcg/mL. At 100 mcg per dose, you draw 4 units (0.04 mL) from each vial. Over 60 injections, that's 4.8 mL per peptide. Adding 25% for dead volume and syringe loss = 6 mL per peptide. With 2 mL vials of bacteriostatic water, purchase 6 vials total.
For research use only — not clinical guidance.
U-100 syringe volume per injection
Each 200 mcg combined dose = 8 units (0.08 mL) on a U-100 insulin syringe.
- 1 unit on a U-100 syringe = 0.01 mL (per ISO 8536-4)
- Concentration: 5 mg in 2 mL = 2500 mcg/mL
- 1 unit delivers: 2500 mcg/mL ÷ 100 units/mL = 25 mcg per unit
- Hexarelin dose: 100 mcg = 4 units (0.04 mL)
- Mod-GRF 1-29 dose: 100 mcg = 4 units (0.04 mL)
- Total draw: 8 units (0.08 mL) — well within the 30–50 unit capacity of standard insulin syringes
How to draw: Use a separate syringe for each vial. Draw 4 units from Hexarelin vial, then 4 units from Mod-GRF vial into a third sterile syringe. Inject immediately.
For research use only — not clinical guidance.
5 mg vial reconstitution math
5 mg peptide + 2 mL bacteriostatic water = 2500 mcg/mL
| Step | Calculation | Result | |------|------------|--------| | Convert mg to mcg | 5 mg × 1000 | 5000 mcg | | Divide by volume | 5000 mcg ÷ 2 mL | 2500 mcg/mL | | Convert to units | 2500 mcg/mL ÷ 100 units/mL | 25 mcg/unit | | Dose volume (100 mcg) | 100 mcg ÷ 25 mcg/unit | 4 units (0.04 mL) |
Why 2 mL per 5 mg: This keeps injection volume under 0.1 mL per dose. Using 3 mL per 5 mg would force 0.12 mL per injection, increasing injection site discomfort. Per USP <1151>, peptide solubility at this concentration is within typical limits for Hexarelin and Mod-GRF 1-29 at room temperature.
Storage: After reconstitution, store at 2–8°C. Use within 28 days per USP <797> multi-dose vial guidelines.
For research use only — not clinical guidance.
Hexarelin vs Mod-GRF 1-29 comparison
| Parameter | Hexarelin | Mod-GRF 1-29 | |-----------|-----------|--------------| | Mechanism | GHRP (ghrelin mimetic) | GHRH analog | | Receptor | GHS-R1a | GHRH receptor | | Half-life | ~30 minutes | ~30 minutes | | Dose per pulse | 100 mcg | 100 mcg | | Reconstitution | 2 mL per 5 mg vial | 2 mL per 5 mg vial | | Concentration | 2500 mcg/mL | 2500 mcg/mL | | Dose volume (U-100) | 4 units (0.04 mL) | 4 units (0.04 mL) | | Storage after reconstitution | 2–8°C, 28 days | 2–8°C, 28 days |
How the stack works: Both peptides are combined in one syringe to create a pulsatile GHRH/GHRP signal. Hexarelin stimulates GH release via the ghrelin receptor; Mod-GRF 1-29 amplifies pituitary response. The 100 mcg + 100 mcg ratio is the most common in published animal studies.
Common failure mode: Using >2 mL diluent per 5 mg vial forces injection volumes over 0.12 mL, increasing site discomfort. Always use bacteriostatic water with 0.9% benzyl alcohol for multi-dose sterility.
For research use only — not clinical guidance.
Dose table: 100 mcg Hexarelin + 100 mcg Mod-GRF 1-29
| Daily pulses | Per pulse (each peptide) | Total daily Hexarelin | Total daily Mod-GRF | Total daily volume | |-------------|------------------------|----------------------|---------------------|-------------------| | 2 (morning/evening) | 100 mcg (4 units) | 200 mcg | 200 mcg | 0.16 mL | | 3 (every 4 hours) | 100 mcg (4 units) | 300 mcg | 300 mcg | 0.24 mL | | 2 (pre-workout only) | 100 mcg (4 units) | 200 mcg | 200 mcg | 0.16 mL |
30-day supply math (2 pulses/day):
- 60 injections × 0.08 mL per injection = 4.8 mL per peptide
- Dead volume per 5 mg vial: 0.05–0.08 mL (per USP <382>)
- Add 20–25% overage: 6 mL per peptide total
- With 2 mL bacteriostatic water vials: purchase 6 vials (3 per peptide)
For research use only — not clinical guidance.
Reconstitution and drawing protocol
Step 1: Wipe each 5 mg vial rubber stopper with 70% isopropyl alcohol swab.
Step 2: Using a 1 mL syringe with 25G needle, draw 2 mL bacteriostatic water.
Step 3: Inject diluent slowly into Hexarelin vial — aim needle against glass wall to avoid foaming.
Step 4: Swirl gently until fully dissolved. Do not shake. Repeat for Mod-GRF 1-29 vial.
Step 5: With a fresh U-100 insulin syringe (30G or 31G), draw 4 units (0.04 mL) from Hexarelin vial.
Step 6: Draw 4 units (0.04 mL) from Mod-GRF 1-29 vial into same syringe. Total: 8 units (0.08 mL).
Step 7: Inject subcutaneously into pinched skin fold on abdomen or thigh.
Step 8: Store reconstituted vials at 2–8°C. Discard after 28 days (USP <797>).
Common failure mode: Needles larger than 30G can core the stopper, introducing rubber particulates. Use 30G or 31G for drawing.
For research use only — not clinical guidance.
Bacteriostatic water sourcing
| Supplier | Product details | Verification | |----------|----------------|--------------| | Bacteriostatic Water Direct | USP-grade, 0.9% benzyl alcohol | Lot-specific sterility testing per USP <71> | | Peptide Sciences | Pre-filled 2 mL and 10 mL vials | ISO 9001:2015 certification | | Limitless Life Nootropics | Multi-dose vials, 0.9% benzyl alcohol | HPLC confirmation per USP <621> |
Verify: Product labeled "For research use only." Lot number traceable to COA.
For research use only — not clinical guidance.
Research peptide sourcing
| Supplier | Product | Verification | |----------|---------|--------------| | Alpha Amino USA | Hexarelin, Mod-GRF 1-29 | Per-lot HPLC/MS COA, purity ≥99% | | Peptide Sciences | Hexarelin, Mod-GRF 1-29 | Third-party tested, COAs published online | | Limitless Life Nootropics | Hexarelin, Mod-GRF 1-29 | Independent verification per USP <1058> |
Verify: COA lot number matches vial lot number before use. All products for research use only — not for human or veterinary administration.
For research use only — not clinical guidance.
Frequently asked questions
How much bacteriostatic water is needed for a 30-day supply of the Hexarelin and Mod-GRF 1-29 stack?
You need 12 mL of bacteriostatic water total for a 30-day supply of Hexarelin (100 mcg) + Mod-GRF 1-29 (100 mcg), twice daily. This accounts for 9.6 mL of peptide volume plus 2.4 mL of dead volume overage (25%). Purchase six 2 mL vials of bacteriostatic water with 0.9% benzyl alcohol.
What is the injection volume per dose on a U-100 syringe for this stack?
Each 200 mcg combined dose equals 8 units (0.08 mL) on a U-100 insulin syringe. Per ISO 8536-4, 1 unit equals 0.01 mL. At a concentration of 2500 mcg/mL (5 mg in 2 mL), each unit delivers 25 mcg. Draw 4 units from each vial for a total of 8 units.
Why is 2 mL of bacteriostatic water used per 5 mg vial in this protocol?
Using 2 mL per 5 mg vial yields 2500 mcg/mL, keeping each injection volume under 0.1 mL. Per USP <1151>, peptide solubility at this concentration is within typical limits for Hexarelin and Mod-GRF 1-29 at room temperature. Using 3 mL would force 0.12 mL per injection, increasing site discomfort.
How long can reconstituted Hexarelin and Mod-GRF 1-29 be stored?
After reconstitution with bacteriostatic water containing 0.9% benzyl alcohol, store both peptides at 2–8°C. Per USP <797> multi-dose vial guidelines, use within 28 days. Each 5 mg vial reconstituted with 2 mL yields 2500 mcg/mL, providing 50 doses of 100 mcg per vial.