Ipamorelin + CJC-1295 No-DAC — Combined Timing and Dose Planner

Published 2026-09-03 · Lyophile Editorial

Ipamorelin + CJC-1295 No-DAC reconstitution calculator

How much bacteriostatic water for a 30-day supply?

For a 30-day supply at 5 mg Ipamorelin and 2 mg CJC-1295 No-DAC per week, you need 20 mg Ipamorelin and 8 mg CJC-1295 No-DAC total. That reconstitutes in 4 mL bacteriostatic water across four 5 mg Ipamorelin vials and four 2 mg CJC-1295 No-DAC vials.

Bacteriostatic water (0.9% benzyl alcohol preserved) maintains antimicrobial effect for 28 days after first puncture per USP <797> beyond-use dating guidance for multi-dose vials at controlled room temperature.

For research use only — not clinical guidance.

CJC-1295 No-DAC half-life vs. DAC — what changes

CJC-1295 with DAC has a plasma half-life of approximately 6-8 days. The biotinyl-DAC moiety binds albumin, which circulates for about 19 days in humans. One injection covers roughly a week.

CJC-1295 No-DAC (Mod GRF 1-29) has a half-life of approximately 30 minutes. Dipeptidyl peptidase IV and other circulating proteases degrade the native 1-29 fragment quickly. Daily or twice-daily injection is required.

The No-DAC form means daily dual draws from two vials — one Ipamorelin, one CJC — combined into a single syringe. Your bacteriostatic water volume per vial needs to be generous enough for accurate U-100 insulin syringe measurement but not so dilute that subcutaneous injection volume becomes excessive.

mcg per unit on a U-100 insulin syringe

A U-100 insulin syringe has 100 units per 1 mL. Each unit equals 0.01 mL (10 microliters). U-100 refers to insulin concentration, not a fixed volume — but the volume math is consistent across all U-100 syringes.

Concentration (mcg/mL) = Total peptide (mcg) ÷ Total diluent (mL)

mcg per unit = Concentration ÷ 100

Example — 5 mg Ipamorelin vial:

• 5000 mcg ÷ 2 mL = 2500 mcg/mL
• 2500 ÷ 100 = 25 mcg per unit

A 250 mcg dose = 10 units. A 500 mcg dose = 20 units.

Example — 2 mg CJC-1295 No-DAC vial:

• 2000 mcg ÷ 2 mL = 1000 mcg/mL
• 1000 ÷ 100 = 10 mcg per unit

A 100 mcg dose = 10 units. A 200 mcg dose = 20 units.

Common error: using 1 mL diluent in a 5 mg vial to keep injection volume low. That gives 5000 mcg/mL = 50 mcg per unit. A 250 mcg dose becomes 5 units — drawable but with almost no margin for syringe dead space or air bubbles. A 100 mcg dose becomes 2 units, which is genuinely difficult to draw accurately even with a fine-gauge needle.

Combined syringe preparation — step by step

Ipamorelin and CJC-1295 No-DAC are compatible in a single syringe for immediate injection. Both are lyophilized peptides reconstituted in bacteriostatic water, both stable at the slightly acidic pH that bacteriostatic water provides. No separate pH adjustment needed.

1. Reconstitute each peptide in its own vial. For a 30-day supply at the protocol above, use 2 mL per 5 mg Ipamorelin vial and 2 mL per 2 mg CJC-1295 No-DAC vial.
2. Draw your CJC-1295 No-DAC dose first. A 100 mcg dose = 10 units.
3. Draw your Ipamorelin dose second. A 250 mcg dose = 10 units at 25 mcg/unit.
4. Total syringe volume: 20 units = 0.2 mL.
5. Inject subcutaneously, rotating sites.

Why draw CJC first: the needle hub holds roughly 1-2 units that never fully mixes. Drawing the smaller-volume peptide first means any residual in the hub is the smaller dose, reducing error if you don't fully clear the needle.

Practical detail most protocols omit: after drawing both peptides, pull back an extra 1-2 units of air into the syringe, invert, and tap to collect the air bubble at the plunger end. This pushes the full volume through the needle without leaving 1-2 units in the hub — 10-25 mcg of peptide you didn't actually inject.

Dose table — 30-day combined protocol

Assumes 5 mg Ipamorelin vial + 2 mL bacteriostatic water (25 mcg/unit) and 2 mg CJC-1295 No-DAC vial + 2 mL (10 mcg/unit). Weekly totals: 5 mg Ipamorelin, 2 mg CJC-1295 No-DAC.

| Dose (Ipamorelin) | Units on U-100 | Dose (CJC No-DAC) | Units on U-100 | Total Syringe Volume | |---|---|---|---|---| | 200 mcg | 8 units | 100 mcg | 10 units | 18 units (0.18 mL) | | 250 mcg | 10 units | 100 mcg | 10 units | 20 units (0.20 mL) | | 300 mcg | 12 units | 150 mcg | 15 units | 27 units (0.27 mL) | | 400 mcg | 16 units | 200 mcg | 20 units | 36 units (0.36 mL) | | 500 mcg | 20 units | 200 mcg | 20 units | 40 units (0.40 mL) |

30-day supply at 250 mcg Ipamorelin / 100 mcg CJC daily: 7.5 mg Ipamorelin and 3 mg CJC-1295 No-DAC. Round up to eight 5 mg Ipamorelin vials and two 2 mg CJC vials — overage covers reconstitution loss and syringe dead space.

Bacteriostatic water volume and peptide degradation over 30 days

The 28-day window from USP <797> applies to the bacteriostatic water's antimicrobial properties — benzyl alcohol evaporates or degrades over time, and each needle puncture introduces potential contamination. Your peptide has a separate stability curve.

Peptides in solution degrade through two primary pathways:

• Hydrolysis — water attacking peptide bonds
• Oxidation — reactive oxygen species modifying side chains

Both are concentration-dependent. A more dilute solution has more water molecules per peptide molecule and more dissolved oxygen per unit volume. A 5 mg vial reconstituted in 1 mL (5000 mcg/mL) generally holds potency longer than the same vial in 5 mL (1000 mcg/mL), assuming identical storage conditions.

The tension: enough volume for accurate U-100 syringe measurements, but not so dilute that the peptide degrades noticeably by day 25. The sweet spot for most research peptides is 2-3 mL per 5 mg vial — enough for 10-unit draws at typical doses, concentrated enough to stay stable for 3-4 weeks refrigerated.

For a 30-day supply, reconstitute fresh every 7-10 days rather than all at once. Four 5 mg vials of Ipamorelin, each reconstituted with 2 mL when needed, keeps peptide concentration high and bacteriostatic water fresh. Same logic for CJC-1295 No-DAC — reconstitute a 2 mg vial weekly rather than a single 8 mg vial for the month.

Sourcing

Bacteriostatic water:

• BAC Water Depot — pre-filled 30 mL multi-dose vials, 0.9% benzyl alcohol, sterile-filtered
• Peptide Waters — 10 mL and 30 mL options, individually packaged
• Medix Solutions — USP-grade, case quantities available

Research peptides:

• Alpha Amino USA — per-lot HPLC/MS COA, US-shipped
• PurePeptides Research — lyophilized in sealed vials, third-party tested
• Research Chem Supply — bulk options, documented purity analysis

For research use only — not clinical guidance.