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Reconstitution math, dose tables, and tools for research peptides.

Tirzepatide Reconstitution Calculator — 5mg, 10mg, 15mg Vials

Published 2026-06-04 · Lyophile Editorial

Tirzepatide Reconstitution Calculator

Enter your vial size and desired diluent volume below. The calculator returns final concentration and syringe-unit equivalents.

Calculator inputs:

Outputs:

Example: 5mg peptide + 2mL diluent. That's 5000 mcg ÷ 2 mL = 2500 mcg/mL. On a U-100 syringe, 1 unit = 0.01 mL, so 1 unit = 25 mcg. 10 units = 250 mcg. 20 units = 500 mcg.

Final Concentration Table for Common Diluent Volumes

| Vial Size | Diluent Volume | Final Concentration | mcg per 1 unit (U-100) | mcg per 10 units | mcg per 20 units | |-----------|----------------|---------------------|------------------------|------------------|------------------| | 5mg | 1mL | 5000 mcg/mL | 50 mcg | 500 mcg | 1000 mcg | | 5mg | 2mL | 2500 mcg/mL | 25 mcg | 250 mcg | 500 mcg | | 5mg | 3mL | 1667 mcg/mL | 16.7 mcg | 167 mcg | 333 mcg | | 10mg | 1mL | 10000 mcg/mL | 100 mcg | 1000 mcg | 2000 mcg | | 10mg | 2mL | 5000 mcg/mL | 50 mcg | 500 mcg | 1000 mcg | | 10mg | 3mL | 3333 mcg/mL | 33.3 mcg | 333 mcg | 667 mcg | | 15mg | 1mL | 15000 mcg/mL | 150 mcg | 1500 mcg | 3000 mcg | | 15mg | 2mL | 7500 mcg/mL | 75 mcg | 750 mcg | 1500 mcg | | 15mg | 3mL | 5000 mcg/mL | 50 mcg | 500 mcg | 1000 mcg |

How to read this table: Find your vial size in the left column, then pick a diluent volume. The right columns give micrograms per syringe unit. Say you've got a 10mg vial and 2mL diluent — that's 50 mcg per unit. Want 250 mcg? Pull 5 units.

Step-by-Step Math for Each Vial Size

5mg Vial

Step 1: Convert to micrograms. 5mg × 1000 = 5000 mcg

Step 2: Choose diluent volume (example: 2mL).

Step 3: Divide peptide by volume. 5000 mcg ÷ 2 mL = 2500 mcg/mL

Step 4: Convert to syringe units. U-100 syringe: 1 unit = 0.01 mL 2500 mcg/mL × 0.01 mL = 25 mcg per unit

Step 5: Calculate syringe volume for your research amount. 250 mcg ÷ 25 mcg/unit = 10 units 500 mcg ÷ 25 mcg/unit = 20 units

Recommended: Go with 2mL diluent. You land on 25 mcg per unit, which keeps the arithmetic clean across the doses you'll actually work with (250–1000 mcg).

10mg Vial

Step 1: Convert to micrograms. 10mg × 1000 = 10000 mcg

Step 2: Choose diluent volume (example: 2mL).

Step 3: Divide peptide by volume. 10000 mcg ÷ 2 mL = 5000 mcg/mL

Step 4: Convert to syringe units. 5000 mcg/mL × 0.01 mL = 50 mcg per unit

Step 5: Calculate syringe volume. 500 mcg ÷ 50 mcg/unit = 10 units 1000 mcg ÷ 50 mcg/unit = 20 units

Recommended: Use 2mL. This is the setup I reach for most. You get 50 mcg per unit, and the syringe volumes sit in the comfortable 10–30 unit range where small errors don't compound.

15mg Vial

Step 1: Convert to micrograms. 15mg × 1000 = 15000 mcg

Step 2: Choose diluent volume (example: 2mL).

Step 3: Divide peptide by volume. 15000 mcg ÷ 2 mL = 7500 mcg/mL

Step 4: Convert to syringe units. 7500 mcg/mL × 0.01 mL = 75 mcg per unit

Step 5: Calculate syringe volume. 500 mcg ÷ 75 mcg/unit ≈ 6.7 units 1000 mcg ÷ 75 mcg/unit ≈ 13.3 units

Those fractional units are a nuisance to draw accurately. So there's a cleaner option.

Alternative: Use 3mL diluent for rounder numbers. 15000 mcg ÷ 3 mL = 5000 mcg/mL = 50 mcg per unit

Syringe-Unit Reference

U-100 insulin syringes: 100 units total, each unit = 0.01 mL.

| Syringe Units | Volume (mL) | |---------------|-------------| | 5 units | 0.05 mL | | 10 units | 0.10 mL | | 20 units | 0.20 mL | | 30 units | 0.30 mL | | 50 units | 0.50 mL | | 100 units | 1.00 mL |

Use a fresh, sterile syringe for every reconstitution.

Common Mistakes to Avoid

Confusing mg and mcg. 1mg = 1000 mcg. A 5mg vial is 5000 mcg, not 5 mcg. Convert to micrograms first, every time — this is the error that wrecks the rest of the math.

Wrong syringe type. U-100 insulin syringes only. Tuberculin syringes and other calibrations read differently, so don't substitute without verifying the markings.

Wrong diluent volume. Stick to 1–3mL. Too much water dilutes past usable accuracy. Too little, and the volumes get too small to measure cleanly.

Dead space. Some syringes hold liquid in the hub. Keep your technique consistent and it stays predictable, though for research work it's usually negligible.

Shaking during reconstitution. Swirl gently. Shaking can denature the peptide.

How to Reconstitute Tirzepatide

  1. Gather supplies: tirzepatide vial, bacteriostatic water, alcohol swabs, U-100 insulin syringe, sterile vial adapter (optional).
  2. Clean vial tops with an alcohol swab. Let dry completely.
  3. Draw diluent into the syringe — use the volume from your calculation, e.g. 2mL for a 5mg vial.
  4. Inject diluent slowly into the tirzepatide vial. Aim the needle at the vial wall, not the powder, to avoid foaming.
  5. Swirl gently until fully dissolved. Do not shake.
  6. Wait 1–2 minutes for complete dissolution. The solution should be clear.
  7. Store at 2–8°C in the refrigerator. Use within 28 days of reconstitution.

Where to Source Bacteriostatic Water

You need sterile bacteriostatic water (0.9% benzyl alcohol). Distilled water and saline are not substitutes.

For research use only — not clinical guidance. This content provides calculation methods and reference data. No dosing recommendations are made. Follow your institution's protocols for handling and disposal. Consult a qualified professional for any medical applications.